Progesterone-binding components of chick oviduct. Biochemical characterization of purified oviduct progesterone receptor B subunit.

Progesterone-binding Components of Chick Oviduct

The cytoplasmic progesterone-binding proteins of chick oviduct are shown to consist of two major 4 S fractions which occur in equal amounts. They are separated from each other by DEAE-cellulose chromatography. Purification of the two components, A and B, was about 800and 3000-fold, respectively, from estrogen-primed chick oviducts following ultracentrifugation, ammonium sulfate precipitation, g...

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Nuclear Binding of Progesterone in Chick Oviduct

1. Varied doses of labelled or unlabelled progesterone were injected into immature chicks which had previously been stimulated with oestrogen. The concentrations of nuclear bound [3H]progesterone were correlated with the effects of the hormone on endogenous RNA polymerase I and II activities in isolated oviduct nuclei. 2. The extent of nuclear localization of [H]progesterone in oviduct (a proge...

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Progesterone receptor in the chick oviduct: an immunohistochemical study with antibodies to distinct receptor components

We performed immunohistochemical studies of chicken oviduct after different fixation procedures, by using antibodies against the progesterone receptor: polyclonal antibodies IgG-G3 against the "8S" form (an oligomere containing progesterone-binding and nonprogesterone-binding units), polyclonal antibodies IgG-RB against the progesterone-binding B subunit, and monoclonal BF4 against the non-prog...

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Progesterone Receptor in the Chick Oviduct: an Immunohistochemical Study with Antibodies to Distinct Receptor Components

We performed immunohistochemical studies of chicken oviduct after different fixation procedures, by using antibodies against the progesterone receptor: polyclonal antibodies IgG-G3 against the "8S" form (an oligomere containing progesterone-binding and nonprogesterone-binding units), polyclonal antibodies IgG-RB against the progesterone-binding B subunit, and monoclonal BF4 against the non-prog...

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Progesterone Receptor in the Chick Oviduct: an Immunohistochemical Study with Antibodies to Distinct Receptor Components

We performed immunohistochemical studies of chicken oviduct after different fixation procedures, by using antibodies against the progesterone receptor: polyclonal antibodies IgG-G3 against the "8S" form (an oligomere containing progesterone-binding and nonprogesterone-binding units), polyclonal antibodies IgG-RB against the progesterone-binding B subunit, and monoclonal BF4 against the non-prog...

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Progesterone-binding components of chick oviduct. Biochemical characterization of purified oviduct progesterone receptor B subunit.

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